Programmable nucleases, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and CRISPR systems, have enabled rapid advances in the …

Encodes ZFHD1, a member of the zinc finger homeodomain transcriptional factor family. Binds to the 62 bp promoter region of ERD1 (early responsive to dehydration stress 1). Expression of ZFHD1 is induced by drought, high salinity and abscisic acid. NEW. Try the new Gene table. Try the new Transcript table.

Addgene is a nonprofit plasmid repository. We store and distribute high-quality plasmids from your colleagues. Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, …

A Better Way toShare Science. A Better Way to. Share Science. Type 3 or more characters for results. We distribute 141,398 plasmids on behalf of 5,950 labs from around the world. Our collection also contains 877 ready-to-use viral vectors and 188 recombinant antibodies. These materials have been requested 2,056,313 times and shipped to 111 ...

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE, Maeder ML, Pearlberg J, Joung JK, Peterson RT, Yeh JR Nat Protoc. 2009. 4(12):1855-67. PubMed Article Plasmids from Article ...

Chloramphenicol. Tetracycline. Ampicillin. Ampicillin. Instructions: To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip, puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate. To patch the hole, use sterile tape or a portion of a fresh aluminum seal.

Human zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain. ... Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications. For your Materials & Methods section ...

Depositing Labs: Scot Wolfe, Nathan Lawson. This plasmid kit contains reagents for engineering zinc finger arrays by modular assembly method. This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from ...

Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, …

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Zinc Finger Consortium Modular Assembly Kit v1.0B Unpublished. Plasmids from Article. No plasmids are currently publicly available from Addgene for this article.

Plasmid pSG5-FLAG-mEKLF Zinc Fingers from Dr. James Bieker's lab contains the insert EKLF and is published in Mol Cell Biol. 2001 May;21(9):3118-25. This plasmid is available through Addgene.

Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene …

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas …

Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Liu Y, Zhao N, Kanemaki MT, Yamamoto Y, Sadamura Y, Ito Y, Tokunaga M, Stasevich TJ, Kimura H. Genes Cells. 2021 Nov;26(11):905-926. doi: 10.1111/gtc.12893.

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Zinc Finger Consortium Modular Assembly Kit v1.0T Unpublished. Plasmids from Article. No plasmids are currently publicly available from Addgene for this article.

Zinc Finger MYND-Type Containing 8 (ZMYND8) is epigenetically regulated in mutant Isocitrate Dehydrogenase 1 (IDH1) glioma to promote radioresistance. Carney SV, Banerjee K, Mujeeb A, Zhu B, Haase S, Varela ML, Kadiyala P, Tronrud CE, Zhu Z, Mukherji D, Gorla P, Sun Y, Tagett R, Nunez FJ, Luo M, Luo W, Ljungman M, Liu Y, Xia Z, Schwendeman …

Because zinc finger arrays (encoded in a 2 × 0.3~0.6 k base pair (kbp) DNA) are small in size, compared to TALE arrays (2 × 1.7~2 kbp) or S. pyogenes Cas9 …

Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene …

Users must order the complementary plasmid Swachmann-Bodian-Diamond Syndrome Gene, sbds_R (OZ546) [Addgene plasmid 28071] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease ...

Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Liu Y, Zhao N, Kanemaki MT, Yamamoto Y, Sadamura Y, Ito Y, Tokunaga M, Stasevich TJ, Kimura H Genes Cells. 2021 Nov;26(11):905-926. doi: 10.1111/gtc.12893. Epub 2021 Sep 20. PubMed Article

Zinc-finger domains designed for the loxF8 flanking sequences were evolved based on the established substrate-linked directed evolution of recombinases 2,4,6,7,8. SLiDE links excision activity of ...

Zinc finger proteins are transcription factors with the finger domain, which plays a significant role in gene regulation. As the largest family of transcription factors in …

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. Polstein LR, Gersbach CA J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. Epub 2012 Sep 27.

Holiday Schedule: Addgene will be closed December 25 - January 1. Order processing and shipping will resume on January 2, 2024. ... Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, Eichtinger M, Jiang T, Foley JE, Winfrey ...

Engineered zinc finger nucleases can stimulate gene targeting at specific genomic loci in insect, plant and human cells. ... strain available through Addgene as part of the Zinc Finger Consortium ...

Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng X, Noyes , Zhu LJ, Lawson ND, Wolfe SA Nat Biotechnol. 2008 Jun . 26(6):695-701.

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Nuclear and mitochondrial DNA editing in human cells with zinc finger deaminases. Lim K, Cho SI, Kim JS Nat Commun. 2022 Jan 18;13(1):366. doi: 10.1038/s41467-022-27962-0. PubMed Article Plasmids from Article. ID

DNA binding site to CCR5 left-handed zinc finger (GTCATCCTCATC) inserted upstream of the CAG promoter. Vector type. Mammalian Expression Growth in Bacteria ... Principal Investigator, cite the article in …

This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene zgc:111821. Please note that this plasmid does NOT contain the zgc:111821 sequence. Users must order the complementary plasmid zgc:111821_R (OZ598) [Addgene plasmid 35238] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted ...

Description CD9 molecule. Also known as BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29. Species Homo sapiens. Entrez ID 928. MGC ID BC011988.

a ZFD (zinc finger deaminase) architecture. Split-DddA tox halves are fused to the C terminus of ZFPs (zinc finger proteins) (C type). b Optimization of the ZFD platform using pTarget libraries ...